Schistosomiasis is a disease caused by the Schistosoma parasite. The parasite is most common in areas of the world with poor sanitation and spreads through contact with contaminated swimming, bathing, or drinking water. Symptoms of schistosomiasis include itchy rash, fever, chills, cough, and muscle ache.
Read more about how schistosomiasis is transmitted, diagnosed, and treated on our Articles page.
The IVD Schistosoma Serum Antibody Detection Microwell ELISA test kit provides accurate, reliable detection of Schistosoma antibodies in human serum samples. Schistosomiasis can be diagnosed using stool or urine samples to detect parasitic eggs; however, as eggs are passed only intermittently and may not be present in all samples, these methods remain less precise. Enzyme-linked immunosorbent assays (ELISA) using Schisotoma-specific antigens have been adopted as an alternative early detection method and have proven to be specific, sensitive, and rapid.
The IVD Schistosoma Serum Antibody Detection Microwell ELISA test is performed in four steps (three incubation periods and a stop solution):
- The first incubation: In this step, antigen attached to the kit microwells capture Schistosoma-specific antibodies present in the serum sample; a washing step removes any unbound samples.
- The second incubation: This step adds Protein A antibody conjugated to horseradish peroxidase, which attaches to any antibodies bound to the microwells; a washing step removes any unbound conjugate.
- The third incubation: This step adds a chromogen, which turns blue in the presence of the enzyme complex and peroxide.
- The addition of a stop solution: This ends the chemical reaction and turns the blue to yellow in the presence of Schistosoma antibodies. If no antibody is captured, or if there is an insufficient antibody level, the solution will remain colorless.
Each test kit contains 96 wells and has up to a 12-month shelf life. Test kits should be stored between 2-8 ˚C. Please contact us for pricing and availability.