Escherichia coli (E. coli) bacteria have several strains and can cause a variety of illnesses. E. coli O157 is a particularly virulent strain of enterohemorrhagic E. coli (EHEC). E. coli O157 passes through the oral-fecal route and often causes food poisoning from undercooked beef and dairy products.
E. coli O157 can lead to hemolytic uremic syndrome (HUS) and hemorrhagic colitis (HC). These conditions can cause abdominal cramps, diarrhea, intestinal bleeding, kidney damage, anemia, excessive clumping of blood platelets, and death.
Read more about how E. coli O157 is transmitted, diagnosed, and treated on our Articles page.
The IVD E. coli O157 Stool Antigen Detection Microwell ELISA test kit provides accurate, reliable detection of E. coli O157 antigens in human stool samples. The enzyme-linked immunosorbent assay (ELISA) for stool samples was developed as a rapid, precise way to detect and diagnose E. coli O157 infection without the need for prior culturing. ELISAs have been shown to be as accurate as traditional methods by experienced laboratory technicians; in addition, they are easy to perform and require no specialized training.
The IVD E. coli O157 Stool Antigen Detection Microwell ELISA test is performed in four steps (three incubation periods and a stop solution):
- The first incubation: In this step, antibodies attached to the kit microwells capture E. coli O157-specific antigens present in the stool sample; a washing step removes any unbound sample.
- The second incubation: This step adds an anti-E. coli O157 antibody conjugated to horseradish peroxidase, which “sandwiches” any antigen bound to the microwells; a washing step removes any unbound conjugate.
- The third incubation: This step adds a chromogen, which turns blue in the presence of the enzyme complex and peroxide.
- The addition of a stop solution: This ends the chemical reaction and turns the blue to yellow in the presence of E. coli O157 antigen. If no antigen is captured, or if there is an insufficient antigen level, the solution will remain colorless.
Each test kit contains 96 wells and has up to a 24-month shelf life. Test kits should be stored between 2-8 ˚C. Please contact us for pricing and availability.