The term “adenovirus” covers a range of viral infections. Adenoviruses can include the common cold, croup, bronchitis, pneumonia, and conjunctivitis (otherwise known as pink eye). Enteric adenoviruses, or adenoviruses associated with the intestines, have been identified as a leading cause of diarrhea in children worldwide. Research has also indicated that adenoviruses are responsible for 4-5% of all hospitalized cases of viral gastroenteritis.
Enteric adenoviruses are believed to be spread via the fecal-oral route. The main symptoms of enteric adenoviruses include diarrhea and vomiting; 40-90% of patients also experience fever.
Read more about how adenoviruses are transmitted, diagnosed, and treated on our Articles page.
The IVD Adenovirus Stool Antigen Detection Microwell ELISA test kit provides accurate, reliable detection of adenovirus antigens in human stool samples. Many labs have traditionally used electron microscopy to diagnose enteric adenoviruses; however, this method is expensive and time-consuming and is not readily available in many areas. Other common diagnostic methods include direct genome proliferation and nucleic acid hybridization, but these methods are neither specific nor rapid. Enzyme linked immunosorbent assays (ELISA) using adenovirus-specific antibodies have been adopted as an alternative detection method and have proven to be specific, sensitive, and rapid.
The IVD Adenovirus Stool Antigen Detection Microwell ELISA test is performed in five steps (four incubation periods and a stop solution):
- The first incubation: In this step, antibodies attached to the kit microwells capture adenovirus-specific antigens present in the stool sample; a washing step removes any unbound samples.
- The second incubation: This step adds a second anti-adenovirus antibody to “sandwich” the antigens; a washing step removes any unbound conjugate.
- The third incubation: This step adds antibodies conjugated to horseradish peroxidase to the “sandwich” from the previous step; a washing step removes any unbound conjugate.
- The fourth incubation: This step adds a chromogen, which turns blue in the presence of the enzyme complex and peroxide.
- The addition of a stop solution: This ends the chemical reaction and turns the blue to yellow in the presence of adenovirus antigen. If no antigen is captured, or if there is an insufficient antigen level, the solution will remain colorless.
Each test kit contains 96 wells and has up to a 24-month shelf life. Test kits should be stored between 2-8 ˚C. Please contact us for pricing and availability.