Verotoxins are caused by a strain of the Escherichia coli bacteria. Infection by verotoxin producing E. coli’s can result in hemorrhagic colitis (HC), a form of gastroenteritis characterized by diarrhea and abdominal cramps. More rarely, verotoxins can cause hemolytic uremic syndrome (HUS), a condition in which infected red blood cells clog and damage the kidneys.
E. coli verotoxin producing strains can be found throughout North and South America, Europe, and Asia, and infection rates tend to be highest during the summer months. The bacteria are food- and water-borne and can also spread from person to person through the fecal-oral route. In healthy adults, infections are rarely serious and typically last only several days. However, infection can be serious or even fatal in infants, young children, and the elderly.
Read more about how Verotoxin is transmitted, diagnosed, and treated on our Articles page.
The IVD E. coli Verotoxin Stool Antigen Detection Microwell ELISA test kit provides accurate, reliable detection of E.coli verotoxin antigens in human stool samples. Diagnosis of infections used to require time-consuming, technically difficult laboratory tests. However, enzyme-linked immunosorbent assays (ELISA) using E. coli vero-specific antibodies have been adopted as an alternative detection method and have proven to be specific, sensitive, and rapid.
The IVD E. coli Verotoxin Stool Antigen Detection Microwell ELISA test is performed in five steps (four incubation periods and a stop solution):
- The first incubation: In this step, antibodies attached to the kit microwells capture E. coli verotoxin-specific antigens present in the stool sample; a washing step removes any unbound samples.
- The second incubation: This step adds an anti-E. coli verotoxin antibody to “sandwich” the antigens; a washing step removes any unbound conjugate.
- The third incubation: This step adds a secondary antibody conjugated to horseradish peroxidase; a washing step removes any unbound conjugate.
- The fourth incubation: This step adds a chromogen, which turns blue in the presence of the enzyme complex and peroxide.
- The addition of a stop solution: This ends the chemical reaction and turns the blue to yellow in the presence of the E. coli veroantigen. If no antigen is captured, or if there is an insufficient antigen level, the solution will remain colorless.
Each test kit contains 96 wells and has up to a 12-month shelf life. Test kits should be stored between 2-8 ˚C. Please contact us for pricing and availability.