Caused by the Campylobacter bacteria, campylobacteriosis is the most common human form of gastroenteritis in the world. Humans generally contract campylobacteriosis by consuming contaminated animal products. While the disease is generally not dangerous, it can be life-threatening for the elderly and young infants, as well as for people with compromised immune systems. It has also been associated with Guillain-Barre Syndrome, a debilitating neurological disease.
Read more about how campylobacteriosis is transmitted, diagnosed, and treated on our Articles page.
The IVD Campylobacter Stool Antigen Detection Microwell ELISA test kit provides accurate, reliable detection of Campylobacter antigens in human stool samples. Traditional diagnosis of campylobacteriosis has involved cultivating the Campylobacter bacteria from a potentially infected sample; this multi-step process is time-consuming, however, and requires a microaerobic environment. Enzyme-linked immunosorbent assays (ELISA) using Campylobacter-specific antibodies have been adopted as an alternative detection method and have proven to be specific, sensitive, and rapid.
The IVD Campylobacter Stool Antigen Detection Microwell ELISA test is performed in four steps (three incubation periods and a stop solution):
- The first incubation: In this step, antibodies attached to the kit microwells capture Campylobacter-specific antigens present in the stool sample; a washing step removes any unbound samples.
- The second incubation: This step adds an anti-Campylobacter antibody conjugated to horseradish peroxidase which “sandwiches” any antigen bound to the microwells; a washing step removes any unbound conjugate.
- The third incubation: This step adds a chromogen, which turns blue in the presence of the enzyme complex and peroxide.
- The addition of a stop solution: This ends the chemical reaction and turns the blue to yellow in the presence of the Campylobacter antigen. If no antigen is captured, or if there is an insufficient antigen level, the solution will remain colorless.
Each test kit contains 96 wells and has up to a 12 month shelf life. Test kits should be stored between 2-8 ˚C. Please contact us for pricing and availability.